approche GRADE (Grading of Recommendations Assessment, Development, and Evaluation) (consulter les tableaux A1 et A2 de l’annexe en ligne). BéNéFICES, RISQUES, COûTS La mise en œuvre des recommandations de la présente directive à l’aide d’outils de dépistage validés et de stratégies d’intervention brève peut améliorer la capacité des fournisseurs de soins obstétricaux à reconnaître la consommation d’alcool et la consommation problématique d’alcool chez les femmes enceintes ou en âge de procréer. Il est attendu des fournisseurs de soins de santé qu’ils deviennent confiants et compétents en matière de prise en charge et de soutien de ces femmes afin qu’elles puissent avoir la meilleure santé possible et une issue de grossesse optimale. DÉCLARATIONS SOMMAIRES (CLASSEMENT GRADE ENTRE PARENTHèSES) RECOMMANDATIONS (CLASSEMENT GRADE ENTRE PARENTHèSES).

To establish national standards of care for screening and counselling pregnant women and women of child-bearing age about alcohol consumption and possible alcohol use disorder based on current best evidence.

Health care providers who care for pregnant women and women of child-bearing age.

Pregnant women and women of child-bearing age and their families.

Medline, EMBASE, and CENTRAL databases were searched for “alcohol use and pregnancy.” The results were filtered for a publication date between 2010 and September 2018. The search terms were developed using Medical Subject Headings terms and keywords, including pre-pregnancy, pregnant, breastfeeding, lactation, female, women, preconception care, prenatal care, fetal alcohol spectrum disorder, prenatal alcohol exposure, drinking behavior, alcohol abstinence, alcohol drinking, binge drinking, alcohol-related disorders, alcoholism, alcohol consumption, alcohol abuse, benzodiazepines, disulfiram, naltrexane, acamprosate, ondansetron, topiramate, cyanamide, en of child-bearing age and those who are pregnant. It is anticipated that health care providers will become confident and competent in managing and supporting these women so they can achieve optimal health and pregnancy outcomes.

RECOMMENDATIONS (GRADE RATINGS IN PARENTHESES).

RECOMMENDATIONS (GRADE RATINGS IN PARENTHESES).The prediction of clonal genetic value for yield is challenging in oil palm (Elaeis guineensis Jacq.). Currently, clonal selection involves two stages of phenotypic selection (PS) ortet preselection on traits with sufficient heritability among a small number of individuals in the best crosses in progeny tests, and final selection on performance in clonal trials. The present study evaluated the efficiency of genomic selection (GS) for clonal selection. The training set comprised almost 300 Deli × La Mé crosses phenotyped for eight palm oil yield components and the validation set 42 Deli × La Mé ortets. SGC 0946 Genotyping-by-sequencing (GBS) revealed 15,054 single nucleotide polymorphisms (SNP). The effects of the SNP dataset (density and percentage of missing data) and two GS modeling approaches, ignoring (ASGM) and considering (PSAM) the parental origin of alleles, were assessed. The results showed prediction accuracies ranging from 0.08 to 0.70 for ortet candidates without data records, depending on trait, SNP dataset and modeling. ASGM was better (on average slightly more accurate, less sensitive to SNP dataset and simpler), although PSAM appeared interesting for a few traits. With ASGM, the number of SNPs had to reach 7,000, while the percentage of missing data per SNP was of secondary importance, and GS prediction accuracies were higher than those of PS for most of the traits. Finally, this makes possible two practical applications of GS, that will increase genetic progress by improving ortet preselection before clonal trials (1) preselection at the mature stage on all yield components jointly using ortet genotypes and phenotypes, and (2) genomic preselection on more yield components than PS, among a large population of the best possible crosses at nursery stage.The δ-aminolevulinic acid (δ-ALA) is an intermediate in the biosynthetic pathway of tetrapyrroles. Tetrapyrroles play vital roles in many biological processes such as photosynthesis, respiration, and light-sensing. ALA-dehydratase (ALAD) combines two molecules of δ-ALA to form porphobilinogen. In citrus, the silencing of ALAD caused discrete yellow spots and necrosis in leaves and stems. Additionally, it caused rapid death in developing new shoots. Herein, we hypothesize that the accumulation of δ-ALA results in severe stress and reduced meristem development. For that reason, we investigated the dynamic changes in the expression profiles of 23 microRNA (miRNA) identified through small RNA sequencing, from CTV-tALAD plants in comparison with healthy C. macrophylla and C. macrophylla infiltrated with CTV-wt. Furthermore, we reported the effect of ALAD silencing on the total phenolics, H2O2, and reactive oxygen species (ROS) levels, to examine the possibilities of miRNAs involving the regulation of these pathways. Our results showed that the total phenolics content, H2O2, and O2- levels were increased in CTV-tALAD plants. Moreover, 63 conserved miRNA members belonging to 23 different miRNA families were differentially expressed in CTV-tALAD plants compared to controls. The identified miRNAs are implicated in auxin biosynthesis and signaling, axillary shoot meristem formation and leaf morphology, starch metabolism, and oxidative stress. Collectively, our findings suggested that ALAD silencing initiates stress on citrus plants. As a result, CTV-tALAD plants exhibit reduced metabolic rate, growth, and development in order to cope with the stress that resulted from the accumulation of δ-ALA. This cascade of events led to leaf, stem, and meristem necrosis and failure of new shoot development.MYB transcription factors (TFs) are one of the largest TF families, and R2R3-type MYB TFs participate in the multiply abiotic stress responses in wheat. In this study, an R2R3-type MYB gene Myb protein colourless 1 located on chromosome D (named TaMpc1-D4), was cloned from wheat. TaMpc1-D4-GFP protein was localized in the nucleus. Overexpression of TaMpc1-D4 reduced drought tolerance in transgenic Arabidopsis lines, which was supported by the lower germination rate, the shorter root length, a higher level of O2- and malonaldehyde (MDA), the decreased proline content, and limited activities of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT). Furthermore, P5CS1, RD29A, RD29B, DREB2A, ABF3, CBF1, CBF2, CBF3, ERF1, POD1, SOD (Cu/Zn), and CAT1 genes related to the stress and antioxidant system were remarkably down-regulated in TaMpc1-D4 transgenic Arabidopsis lines under drought stress. Silencing TaMpc1-D4 expression in wheat enhanced the relative water content (RWC), the proline content, and the activities of antioxidant enzymes, and activated stress-related and antioxidant-related genes (DREB1, DREB3, ERF3, ERF4b, ABF, P5CS, POD, SOD (Fe), and CAT).